alcian blue staining solution  (Servicebio Inc)

Journal: Scientific Reports

Article Title: Akkermansia muciniphila affects colitis by inhibiting ferroptosis signaling pathway

doi: 10.1038/s41598-026-38452-4

Figure Lengend Snippet: A. muciniphila could improve colitis and it was associated with ferroptosis. ( A ) Construction of mouse colitis model (from left to right: weight gain rate; stool shape score; fecal occult blood score). n = 6. ( B ) HE staining of mouse colon tissues. n = 3. ( C ) The number of goblet cells in colon tissues observed by alcian blue staining (black arrows indicate goblet cells). n = 3. ( D ) Transmission electron microscopy of mouse colon tissues (red arrows indicate endoplasmic reticulum, blue arrows indicate mitochondria). n = 3. (E)ELISA of mouse colon tissues. n = 6. ( F ) Western blot detection of mouse colon tissues. n = 3. AKK, Akkermansia muciniphila ; MDA, malondialdehyde; ROS, reactive oxygen species; GSH, glutathione.

Article Snippet: The slices of mice colon tissues were baked, dewaxed, hydrated, and stained with alcian blue staining solution (Servicebio, G1027-100 mL) for 15–30 min.

Techniques: Staining, Transmission Assay, Electron Microscopy, Enzyme-linked Immunosorbent Assay, Western Blot

Journal: Stem Cell Research & Therapy

Article Title: Immortalized human hair follicle-derived mesenchymal-like stromal cells for the long-term production of scalable Immunomodulatory and regenerative secretome

doi: 10.1186/s13287-025-04775-8

Figure Lengend Snippet: iHF-MSC immortalization and phenotyping. A SV40LT mRNA expression by RT-PCR. GAPDH was employed as a reference gene. Positive Control (C+): SV40T plasmid. Negative Control (C-): non-infected HF-MSCs. B Flow cytometry histograms for iHF-MSC surface markers pre-gated on live cells after doublet discrimination. Percentages shown correspond to the proportion of positive cells for the populations indicated in the legend (in order: isotype, HF-MSCs, C18, C20, C26, C39). Histograms shown are representative of one replicate. Y-axis: Count. C iHF-MSC differentiation ability. Optical micrographs of Alizarin Red S staining for osteogenic differentiation, Oil Red O staining for adipogenic differentiation, and Alcian Blue staining for chondrogenic differentiation. D Epifluorescence microscopy images of CD56 immunostaining (DAPI in blue, CD56 in green). Scale bars = 20 μm. Data are representative of 3 independent experiments with 4 replicates per group. Abbreviations: HF-MSCs: hair follicle-derived mesenchymal stromal cells. iHF-MSCs: immortalized HF-MSCs. C18: clone 18. C20: clone 20. C26: clone 26. C39: clone 39. CD: Cluster of Differentiation. MHC-II: major histocompatibility complex class II

Article Snippet: After 14 days of osteogenic and adipogenic induction, and 21 days of chondrogenic induction, cells were fixed in Saccomanno Fixation Solution for 30 min and stained with the corresponding dye: Alizarin Red S staining solution (Merck, TMS008) for osteogenic differentiation, OilRed staining solution (Merck, O1391) for adipogenic differentiation and Alcian Blue staining solution (Merck, TMS010) for chondrogenic differentiation.

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Positive Control, Plasmid Preparation, Negative Control, Infection, Flow Cytometry, Staining, Epifluorescence Microscopy, Immunostaining, Derivative Assay, Immunopeptidomics